Can i autoclave buffers

For example, a 0. Adjust the volume to 3 liters with H2O. Dispense into aliquots. Sterilize by autoclaving. The final concentrations of the ingredients are 3. Store the solution at 4 C and transfer it to an ice bucket just before use. Measure Mix the solution well and then sterilize by passing it through a 0. Store the sterile freezing medium at a controlled room temperature C. EDTA 0. Autoclaved water and sterile stock combined in a hood should still be sterile.

You might worry about evaporation though -- everytime you heat the buffer, you're going to lose some amount to steam because you can't tightly cap the bottle while it's in the autoclave or immediately after.

Whether it's a trivial amount or not, I don't know. Further heating converts the carbonate into the oxide at ca. I doubt it would be harmed and wouldn't hesitate to do it if I had to, but assuming you have access to a TC hood or similar, wouldn't it be easier to just remove some working solution in the hood from the bottle each time you want it while leaving your stock sterile? Or, if you don't have access to a hood, flaming the entrance and pouring might be good enough "sterility", depending on your needs.

Or filter-sterilizing. Re-autoclaving seems excessive, if fairly harmless. I have seen similar solutions autoclaved multiple times with no bad effects. But I think that a terminal. NaHCO 3 in aqueous solution "begins to break up into carbon dioxide and sodium carbonate at about 20C and completely on boiling. I would assume that anything that is stable enough to be autoclaved in the first place can be autoclaved again. If you're worried about evaporation, maybe do some checks to see how much you're losing each time.

Bunny Wigglesworth at PM on July 6, However, before autoclaving any solution you should always check whether it contains any heat-labile ingredients Media Recipes. If it does, the heat-labile substance will usually have to be prepared separately, filter-sterilized, and added to the remainder of the solution after autoclaving.

Also, certain combinations of compounds that are stable when autoclaved on their own can react at the high temperatures in the autoclave to produce compounds that inhibit microbial growth e. Pipet tips, microfuge tubes, and their storage containers generally are autoclavable, if unsure please ask about other plastics before you experiment and make a mess. General autoclave use: Leave at least a quarter of the container volume as free space otherwise your solutions will boil over.

Place containers in autoclavable pans to catch liquids from any breaking containers. Make sure all caps are loose and taped on at least on one side including tinfoil caps Use autoclave tape to tape on caps, also put a small bit of autoclave tape on any other items you autoclave. The tape will change color during autoclaving, thus signaling to any future user that the item has really been sterilized.

Write in the date, contents and weight of your item s , pressure and time used, principal investigator, and initials.